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Hydrogen peroxide (H2O2) is a chemical compound with strong oxidizing capabilities, which acts against a variety of microorganisms, including bacteria, viruses, fungi, yeasts and spores1,2. It has been utilized in several formulations of bleaching agents, disinfectants and sanitizers.

The concentration of H2O2 differs according to the objective of the formulation1. While a concentration of 3% H2O2 is a potent disinfectant, up to 0.5% concentration of H2O2 is thought to be effective against surface viruses2.

The low concentration of H2O2 is included in formulations to help terminate bacterial spores that contaminate a solution3. For this application, H2O2 content is established with redox titration methods utilizing cerium (IV) sulfate as a titrant and DMi140-SC electrode.

1. Titrant: Cerium sulfate, Ce(SO4)2, 0.1 mol/L

H2O2 + 2Ce(SO4)2 → Ce2(SO4)3 + H2SO4 + O2

1.  Titer determination of Cerium sulfate      c(Ce(SO4)2) = 0.1 mol/L      Titer value obtained = 1.00505      s = 0.00172      srel = 0.171%      n = 6

(a) Standard 1.5% H2O2 solution:

Table 1. Source: Mettler Toledo - Titration

Table 2. Source: Mettler Toledo - Titration

The H2O2 content in disinfectant and sanitizer samples is established and values acquired by redox titration with cerium sulfate are in sound agreement with the content of H2O2 anticipated in both samples. The repeatability achieved for standard as well as sample is excellent in terms of comparable standard deviation, i.e., srel.

Precipitate cerium with NaOH, filter, and handle as special waste.

http://www.mt.com/ch/en/home/products/Laborat ory_Analytics_Browse/Product_Family_Browse_titr ators_main/Titration_Excellence.html?cmp=als_Tit ration-Excellence

Table 3. Source: Mettler Toledo - Titration

Table 4. Source: Mettler Toledo - Titration

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Last updated: Jan 21, 2021 at 6:02 AM

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